Evaluation of the effectiveness of an entomopathogenic consortium for the control of mealybug (Planococcus citri) located on the María Auxiliadora campus of the Salesian Polytechnic University
DOI:
https://doi.org/10.56294/evk2024123Keywords:
Entomopathogenic Consortium, Planoccocus Citri, Biological Control, Invertebrates, BioinsecticideAbstract
Introduction: in the city of Guayaquil, the mealybug pest poses a threat to various plant species. Biological control strategies, such as the use of one of its natural predators, ladybugs, are also possible, as are other solutions such as the use of entomopathogenic fungi.
Objective: to evaluate the efficacy of an entomopathogenic consortium consisting of the species Beauveria bassiana, Metarhizium anisopliae, Lecanicillium lecanii, and Purpureocillium lilacinum against the mealybug species Planococcus citri in lemon plants.
Method: the experimental strategy was carried out on the grounds of the Salesian Polytechnic University, María Auxiliadora campus. Lemon trees were infected with Planococcus citri for 4 to 6 months, two weeks before the application of the treatments. The 7 treatments, except for the application control, consisted of 3 different concentrations (200 %, 100 %, and 50 %) of the positive control (chemical detergent) and the entomopathogenic consortium.
Results: the mortality rate of scale insects and the efficacy of the consortium were analyzed. Treatment 3 (100%) produced the highest mortality rate; while treatment 2, the positive control, and treatment 3, the entomopathogenic consortium, showed no significant differences in efficacy. In addition, chlorophyll was analyzed before and after treatment; treatment 3 showed significant differences compared to the positive control treatments.
Conclusions: the entomopathogenic fungal consortium proved to be an effective alternative to chemical insecticides for controlling Planococcus citri, as higher mortality of the pest and a significant population reduction were observed with the use of this treatment compared to the negative and positive controls.
References
1. Ghaffari S, Karimia J, Kamali S, Mahdikhani Moghadam E. Biocontrol of Planococcus citri (Hemiptera: Pseudococcidae) by Lecanicillium longisporum and Lecanicillium lecanii in laboratory and greenhouse conditions. J Asian Pac Entomol. 2017;20(2):605-12. DOI: https://doi.org/10.1016/j.aspen.2017.03.019
2. Modafferi A, Ricupero M, Mostacchio G, Latella I, Zappalà L, Palmeri V, et al. Bioactividad de la nanoemulsión a base de aceite esencial de Allium sativum contra Planococcus citri y su depredador Cryptolaemus montrouzieri. Ind Crops Prod. 2024;208. DOI: https://doi.org/10.1016/j.indcrop.2023.117837
3. Mansour R, Belzunces LP, Suma P, Zappalà L, Mazzeo G, Grissa-Lebdi K, et al. Vine and citrus mealybug pest control based on synthetic chemicals. A review. Agron Sustain Dev. 2018;38(37):1-18. DOI: https://doi.org/10.1007/s13593-018-0513-7
4. Herrick NJ, Cloyd RA, Raudenbush AL. Systemic insecticide applications: Effects on citrus mealybug (Hemiptera: Pseudococcidae) populations under greenhouse conditions. J Econ Entomol. 2019;112(4):1048-54. DOI: https://doi.org/10.1093/jee/toy352
5. Zambrano A. Gracias al tratamiento contra la cochinilla, los árboles de Urdesa recuperan su follaje. Alcaldía de Guayaquil. 2023.
6. Khan S, Guo I, Maimaiti Y, Mijit M, Qiu D. Hongos entomopatógenos como agente de biocontrol microbiano. Biotechnol Mol Plant Breed. 2012;3:63-79.
7. Valbuena Puentes AH, Galindo Soracá AM, Boyacá Quintana YM. Efecto del hongo entomopatógeno Beauveria bassiana (balsamo) Vuillemin en el control de la oveja ked (Melophagus ovinos). Rev Investig Vet Peru. 2021;32(2):e18362. DOI: https://doi.org/10.15381/rivep.v32i2.18362
8. Acheampong MA, Coombes CA, Moore SD, Hill MP. Temperature tolerance and humidity requirements of select entomopathogenic fungal isolates for future use in citrus IPM programmes. J Invertebr Pathol. 2020;174:107436. DOI: https://doi.org/10.1016/j.jip.2020.107436
9. Gómez Ramírez H, Zapata Granja A, Torres Del Águila E, Tenorio Cantoral M. Manual de producción y uso de hongos entomopatógenos. Laboratorio de Entomopatógenos SCB - SENASA; 2014.
10. Shin H, Lee S, Lee J. Molecular mechanisms of fungal entomopathogens. J Microbiol Biotechnol. 2020;30(10):2129-40.
11. Abdollahdokht D, Gao Y, Faramarz S, Ghasemi H. Conventional agrochemicals towards nano-biopesticides: an overview on recent advances. Chem Biol Technol Agric. 2022;9(2):120. DOI: https://doi.org/10.1186/s40538-021-00281-0
12. Laudani F, Campolo O, Caridi R, Latella I, Modafferi A, Palmeri V, et al. Based Nano-Insecticide. Insects. 2022;13(12):1150. DOI: https://doi.org/10.3390/insects13121150
13. Carvalho MMP, Corrêa Reis LA, Pinheiro MLC, Moreira MM, Vieira DA, Souza B. Is a diet of Planococcus citri nymphs and adults suitable for Chrysoperla externa for use in biological control?. Rev Bras Entomol. 2023;67(1):e20220010. DOI: https://doi.org/10.1590/1806-9665-rbent-2022-0010
14. Daane KM, Almeida RPP, Bell VA, Walker JTS, Botton M, Fallahzadeh M, et al. Biology and Management of Mealybugs in Vineyards. In: Arthropod Management in Vineyards. Dordrecht: Springer; 2012. p. 271-307. DOI: https://doi.org/10.1007/978-94-007-4032-7_12
15. Mani M, Shivaraju C. Mealybugs and their management in agricultural and horticultural crops. Springer; 2016. DOI: https://doi.org/10.1007/978-81-322-2677-2
16. Kim BS, Park Min-Goo, Gil-Hah Kim, Jeong-Oh Yang. Development of a Concurrent Treatment Technique of Ethyl Formate and Mixtures (Nitrogen, Phosphine) to Control Citrus Mealybug (Planococcus citri). Insects. 2023;14(9):720. DOI: https://doi.org/10.3390/insects14090720
17. Azizi F, Ghasemi R, Ardalan M. Dos errores comunes al aplicar la prueba ANOVA: Guía para investigadores biológicos. Preprints. 2022.
18. Da Costa Stuart AK, Furuie JL, Cataldi TR, Stuart RM, Zawadneak MAC, Labate CA, et al. Metabolomics of the interaction between a consortium of entomopathogenic fungi and their target insect: Mechanisms of attack and survival. Pestic Biochem Physiol. 2023;191:105369. DOI: https://doi.org/10.1016/j.pestbp.2023.105369
19. Naveeda S. Microbial consortium for the management of insect pest pf bitter bround (Momordica charantia L.). [Vellayani]: Department of Agricultural Entomology College of Agriculture; 2018.
20. Cloyd R, Herrick N. Are Entomopathogenic Fungal-based Insecticides and Insect Growth Regulator Mixtures Effective Against the Citrus Mealybug, Planococcus citri (Hemiptera: Pseudococcidae), Feeding on Coleus, Solenostemon scutellarioides, Plants under Greenhouse Conditions?. HortScience. 2023;58(10):1225–9. DOI: https://doi.org/10.21273/HORTSCI17291-23
21. Góngora CE, Gil-Palacio ZN. Control biológico de cochinillas de las raíces del café con hongos entomopatógenos. Cenicafe. 2020;71(2):53–65. DOI: https://doi.org/10.38141/10778/71204
22. Karaca G, Kayahan A, Şimşek B, Karaca İ. Effects of some entomopathogenic fungi on Citrus Mealybug Planococcus citri (Risso) (Hemiptera: Pseudococcidae). Entomologica. 2016;47:39-44.
23. Mohesh M, Basumatary M. Estimation of the chlorophyll concentration in seven Citrus species of Kokrajhar district, BTAD, Assam, India. Trop Plant Res. 2018;5(1). DOI: https://doi.org/10.22271/tpr.2018.v5.i1.012
24. Huang J, Zhang P, Zhang J, Lu Y, Huang F, Li M. Chlorophyll Content and Chlorophyll Fluorescence in Tomato Leaves Infested With an Invasive Mealybug,Phenacoccus solenopsis (Hemiptera: Pseudococcidae). Environ Entomol. 2013;42(5):973-9. DOI: https://doi.org/10.1603/EN12342
25. Veloz-Badillo GM, Riveros-Ramírez J, Angel-Cuapio A, Arce-Cervantes O, Flores-Chávez B, Espitia-López J, et al. The endophytic capacity of the entomopathogenic fungus Beauveria bassiana caused inherent physiological response in two barley (Hordeum vulgare) varieties. 3 Biotech. 2019;9(1):12. DOI: https://doi.org/10.1007/s13205-018-1548-9
26. Greenfield M, Gómez-Jiménez MI, Ortiz V, Vega FE, Kramer M, Parsa S. Beauveria bassiana and Metarhizium anisopliae endophytically colonize cassava roots following soil drench inoculation. Biol Control. 2016;95:40–8. DOI: https://doi.org/10.1016/j.biocontrol.2016.01.002
27. González–Guzmán A, Sacristán D, Sánchez‐Rodríguez AR, Barrón V, Torrent J, Del Campillo MC. Soil Nutrients Effects on the Performance of Durum Wheat Inoculated with Entomopathogenic Fungi. Agronomy. 2020;10(4):589. DOI: https://doi.org/10.3390/agronomy10040589
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